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The influence of the experimental stress on homocysteine plasma levels in rat

Elena Albu, Cristina Filip, Nina Zamosteanu, Irina M. Jaba, Nastasia Gheorghita, Luminita Jerca, O. C. Mungiu
Abstract: Nowadays it is generally accepted that in the aging processes an important role is played by stress. The main mechanism through which stress is involved in the aging processes seems to be the reactive species generation. On the other hand, it is also known that hyperhomocysteinemia is a risk factor in cardiovascular diseases, as it disturbs the normal endothelium functions and generates thrombosis. The mechanism through which homocysteine triggers these eff ects is not yet clarifi ed, but it is believed that a possible explanation is the reactive species involvement. In our work we have studied the influence of experimentally induced stress on homocysteine levels, in rats. Experimental stress was induced by reversing the normal day/night cycle (18oo - 6oo light). The activities of intracellular superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant status (TAS) were measured in order to establish if stress by itself generates reactive species. The plasmatic concentrations of homocysteine were also measured. Our data show an increase in SOD activity, simultaneously with the decrease in GPx activity. The total antioxidant status and homocysteine levels have registered no signifi cant changes. In conclusion, subacute stress activated the antioxidant defense systems, but did not infl uence the total antioxidant capacity and homocysteine levels, in rats.

Keywords: hyperhomocysteinemia, stress, total antioxidant status

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